defined trypsin inhibitor dti human (Thermo Fisher)
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defined trypsin inhibitor dti human
Defined Trypsin Inhibitor Dti Human, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypsin+inhibitor+dti/trypsin+inhibitor/pmc12214878-81-12-15
Average 90 stars, based on 1 article reviews
Defined Trypsin Inhibitor Dti Human, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/trypsin+inhibitor+dti/trypsin+inhibitor/pmc12214878-81-12-15
Average 90 stars, based on 1 article reviews
defined trypsin inhibitor dti human - by Bioz Stars,
2026-09
90/100 stars
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Single Cell:Article Title: Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing Article Snippet: Pluripotent stem cell culture and cryopreservation practices The hPSC cultures were routinely maintained in feeder-free culture conditions on CellBIND® well plates (#3335, Corning®) coated with 0.55 μg/cm2 recombinant laminin 521 (L521) (#LN521; Biolamina, Sweden) in Essential 8TM Flex Medium (E8 Flex) (#A2858501; GibcoTM, Thermo Fisher Scientific), following the weekend-free workflow from the manufacturer. .. In brief, single-cell passaging onto new L521 coatings was carried out with TrypLE SelectTM Enzyme (#12563029) and Defined Article Title: Development of a kidney microphysiological system hardware platform for microgravity studies Article Snippet: PTEC cultures were maintained serum-free in DMEM/F12 (Gibco, Grand Island, NY, Cat. #11330-032) supplemented with 1x insulin-transferrin-selenium-sodium pyruvate (ITS-A, Gibco, Cat. #51300044), 50 nM hydrocortisone (Sigma, St. Louis, MO, Cat. # H6909), and 1x Antibiotic-Antimycotic (Gibco, Cat. # 15240062). .. Upon reaching 75-80% confluence, PTECs were passaged by enzymatic digestion with 0.05% trypsin EDTA (Gibco, Cat. #25200056) and manual cell scraping to obtain a single-cell suspension which was subsequently neutralized with defined Article Title: Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing Article Snippet: Pluripotent stem cell culture and cryopreservation practices The hPSC cultures were routinely maintained in feeder-free culture conditions on CellBIND® well plates (#3335, Corning®) coated with 0.55 μg/cm2 recombinant laminin 521 (L521) (#LN521; Biolamina, Sweden) in Essential 8TM Flex Medium (E8 Flex) (#A2858501; GibcoTM, Thermo Fisher Scientific), following the weekend-free workflow from the manufacturer. .. In brief, single-cell passaging onto new L521 coatings was carried out with TrypLE SelectTM Enzyme (#12563029) and Defined Passaging:Article Title: Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing Article Snippet: Pluripotent stem cell culture and cryopreservation practices The hPSC cultures were routinely maintained in feeder-free culture conditions on CellBIND® well plates (#3335, Corning®) coated with 0.55 μg/cm2 recombinant laminin 521 (L521) (#LN521; Biolamina, Sweden) in Essential 8TM Flex Medium (E8 Flex) (#A2858501; GibcoTM, Thermo Fisher Scientific), following the weekend-free workflow from the manufacturer. .. In brief, single-cell passaging onto new L521 coatings was carried out with TrypLE SelectTM Enzyme (#12563029) and Defined Article Title: Deciphering the heterogeneity of differentiating hPSC-derived corneal limbal stem cells through single-cell RNA sequencing Article Snippet: Pluripotent stem cell culture and cryopreservation practices The hPSC cultures were routinely maintained in feeder-free culture conditions on CellBIND® well plates (#3335, Corning®) coated with 0.55 μg/cm2 recombinant laminin 521 (L521) (#LN521; Biolamina, Sweden) in Essential 8TM Flex Medium (E8 Flex) (#A2858501; GibcoTM, Thermo Fisher Scientific), following the weekend-free workflow from the manufacturer. .. In brief, single-cell passaging onto new L521 coatings was carried out with TrypLE SelectTM Enzyme (#12563029) and Defined Purification:Article Title: Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus Article Snippet: After staining purified T cells with PE anti-CD138 antibody, TCRβ+CD138- and TCRβ+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). .. Depending on the experimental objective, purified TCRβ+CD138+ and TCRβ+CD138cells were stimulated with 1 μg/ml anti-CD3 and -CD28 antibodies (BD Pharmingen), 10 ng/ml PMA and 100 ng/ml ionomycin, 100 mg/ml Collagenase I, 100 mg/ml Collagenase D, 2.5 mg/ml trypsin, 10 μM leupeptin, 500 μM amino-phenyl mercuric acetate (APMA) (all from Sigma-Aldrich, St. Louis, MO), 10 μM FAK inhibitor 14 (Cayman chemicals, Ann Arbor, Michigan), 10 μg/ml MMP9, TrypLE or Defined Expressing:Article Title: Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus Article Snippet: After staining purified T cells with PE anti-CD138 antibody, TCRβ+CD138- and TCRβ+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). .. Depending on the experimental objective, purified TCRβ+CD138+ and TCRβ+CD138cells were stimulated with 1 μg/ml anti-CD3 and -CD28 antibodies (BD Pharmingen), 10 ng/ml PMA and 100 ng/ml ionomycin, 100 mg/ml Collagenase I, 100 mg/ml Collagenase D, 2.5 mg/ml trypsin, 10 μM leupeptin, 500 μM amino-phenyl mercuric acetate (APMA) (all from Sigma-Aldrich, St. Louis, MO), 10 μM FAK inhibitor 14 (Cayman chemicals, Ann Arbor, Michigan), 10 μg/ml MMP9, TrypLE or Defined FACS:Article Title: Circulating CD138 enhances disease progression by augmenting autoreactive antibody production in a mouse model of systemic lupus erythematosus Article Snippet: After staining purified T cells with PE anti-CD138 antibody, TCRβ+CD138- and TCRβ+CD138+ cells were further separated with anti-PE magnetic microbeads (Miltenyi Biotec, Auburn, CA). .. Depending on the experimental objective, purified TCRβ+CD138+ and TCRβ+CD138cells were stimulated with 1 μg/ml anti-CD3 and -CD28 antibodies (BD Pharmingen), 10 ng/ml PMA and 100 ng/ml ionomycin, 100 mg/ml Collagenase I, 100 mg/ml Collagenase D, 2.5 mg/ml trypsin, 10 μM leupeptin, 500 μM amino-phenyl mercuric acetate (APMA) (all from Sigma-Aldrich, St. Louis, MO), 10 μM FAK inhibitor 14 (Cayman chemicals, Ann Arbor, Michigan), 10 μg/ml MMP9, TrypLE or Defined Recombinant:Article Title: Modeling neuroinflammatory interactions between microglia and astrocytes in a human iPSC-based coculture platform Article Snippet: .. The iPSC line was expanded in feeder-free culture on 0.6 μg/cm 2 recombinant human laminin-521 (LN521, BioLamina) in Essential8TM Flex media (E8 flex, Thermo Fisher Scientific), and passaged with TrypLETM Select Enzyme and Defined Article Title: Human iPSC-based coculture model reveals neuroinflammatory crosstalk between microglia and astrocytes Article Snippet: .. The iPSC line was expanded in feeder-free culture on 0.6 μg/cm 2 recombinant human laminin-521 (LN521, BioLamina) in Essential8TM Flex media (E8 flex, Thermo Fisher Scientific), and passaged with TrypLETM Select Enzyme and Defined Article Title: Modeling neuroinflammatory interactions between microglia and astrocytes in a human iPSC-based coculture platform. Article Snippet: .. The iPSC line was expanded in feeder-free culture on 0.6 μg/cm2 recombinant human laminin-521 (LN521, BioLamina) in Essential8TM Flex media (E8 flex, Thermo Fisher Scientific), and passaged with TrypLETM Select Enzyme and Defined In Vitro:Article Title: Clinically compliant cryopreservation of differentiated retinal pigment epithelial cells. Article Snippet: Cells were passaged with TrypLE Select (12563011; Thermo Fisher Scientific, Waltham, MA, USA) after one wash with phosphate-buffered saline (PBS) without Ca2+ and Mg2+ (A1285601; Thermo Fisher Scientific) incubated for 5 min at 37°C, 5% CO2/5% O2. .. TrypLE Select is deactivated with an equal volume of Defined Cell Culture:Article Title: Clinically compliant cryopreservation of differentiated retinal pigment epithelial cells. Article Snippet: Cells were passaged with TrypLE Select (12563011; Thermo Fisher Scientific, Waltham, MA, USA) after one wash with phosphate-buffered saline (PBS) without Ca2+ and Mg2+ (A1285601; Thermo Fisher Scientific) incubated for 5 min at 37°C, 5% CO2/5% O2. .. TrypLE Select is deactivated with an equal volume of Defined Article Title: Development of a kidney microphysiological system hardware platform for microgravity studies Article Snippet: PTEC cultures were maintained serum-free in DMEM/F12 (Gibco, Grand Island, NY, Cat. #11330-032) supplemented with 1x insulin-transferrin-selenium-sodium pyruvate (ITS-A, Gibco, Cat. #51300044), 50 nM hydrocortisone (Sigma, St. Louis, MO, Cat. # H6909), and 1x Antibiotic-Antimycotic (Gibco, Cat. # 15240062). .. Upon reaching 75-80% confluence, PTECs were passaged by enzymatic digestion with 0.05% trypsin EDTA (Gibco, Cat. #25200056) and manual cell scraping to obtain a single-cell suspension which was subsequently neutralized with defined Suspension:Article Title: Development of a kidney microphysiological system hardware platform for microgravity studies Article Snippet: PTEC cultures were maintained serum-free in DMEM/F12 (Gibco, Grand Island, NY, Cat. #11330-032) supplemented with 1x insulin-transferrin-selenium-sodium pyruvate (ITS-A, Gibco, Cat. #51300044), 50 nM hydrocortisone (Sigma, St. Louis, MO, Cat. # H6909), and 1x Antibiotic-Antimycotic (Gibco, Cat. # 15240062). .. Upon reaching 75-80% confluence, PTECs were passaged by enzymatic digestion with 0.05% trypsin EDTA (Gibco, Cat. #25200056) and manual cell scraping to obtain a single-cell suspension which was subsequently neutralized with defined Centrifugation:Article Title: Development of a kidney microphysiological system hardware platform for microgravity studies Article Snippet: PTEC cultures were maintained serum-free in DMEM/F12 (Gibco, Grand Island, NY, Cat. #11330-032) supplemented with 1x insulin-transferrin-selenium-sodium pyruvate (ITS-A, Gibco, Cat. #51300044), 50 nM hydrocortisone (Sigma, St. Louis, MO, Cat. # H6909), and 1x Antibiotic-Antimycotic (Gibco, Cat. # 15240062). .. Upon reaching 75-80% confluence, PTECs were passaged by enzymatic digestion with 0.05% trypsin EDTA (Gibco, Cat. #25200056) and manual cell scraping to obtain a single-cell suspension which was subsequently neutralized with defined |